Journal: The Journal of investigative dermatology

Article Title: Cytokine expression is downregulated by collagen-polyvinylpyrrolidone in hypertrophic scars.

doi: 10.1046/j.1523-1747.1998.00329.x

Figure Lengend Snippet: Figure 6. Immunolocalization of PDGF in human skin sections frozen with the immunoperoxidase technique (avidin-biotin-peroxidase system). (a) Photomicrograph of negative control; (b) photomicrograph of normal skin; (c) photomicrograph of HSc, arrows indicate immunoreactive cells; (d) photomicrograph of HSc treated with collagen-PVP. Scale bar: 200 µm.

Article Snippet: Immunohistochemical procedures were assessed by blocking with 3% egg albumin (Sigma), except for the TGF-β1 assay in which the blockade was done with 3% bovine albumin (Sigma), and then the sections were incubated with a mouse anti-human E-selectin (ELAM-1) or vascular cell-adhesion molecule (VCAM-1) monoclonal IgG at 25 μg (Genzyme, Cambridge, MA) per ml, or a goat anti-human IL-1β, TNF-α, or PDGF polyclonal IgG at 20 μg (R&D Systems, Minneapolis, MN) per ml.

Techniques: Avidin-Biotin Assay, Negative Control

Journal: The Journal of investigative dermatology

Article Title: Cytokine expression is downregulated by collagen-polyvinylpyrrolidone in hypertrophic scars.

doi: 10.1046/j.1523-1747.1998.00329.x

Figure Lengend Snippet: Figure 7. Collagen-PVP effect on pro-inflammatory cytokine expression in HSc. The percentage of cytokine expressing immunoreactive cells was determined for normal skin, HSc, and collagen-PVP treated HSc. Cytokine values were compared between treated and untreated samples as mentioned in Materials and Methods. They were different in (a) spread cells (IL-1β, p 5 0.036; TNF-α, p 5 0.036, and PDGF, p 5 0.003) and (b) blood vessels (IL-1β, p 5 0.002, TNF-α, p , 0.001 and PDGF, p 5 0.01, treated versus untreated HSc). Results represent the mean 6 SEM of at least two sections of each tissue from normal skin (n 5 3), HSc (n 5 4), and HSc previously treated with collagen- PVP (n 5 5).

Article Snippet: Immunohistochemical procedures were assessed by blocking with 3% egg albumin (Sigma), except for the TGF-β1 assay in which the blockade was done with 3% bovine albumin (Sigma), and then the sections were incubated with a mouse anti-human E-selectin (ELAM-1) or vascular cell-adhesion molecule (VCAM-1) monoclonal IgG at 25 μg (Genzyme, Cambridge, MA) per ml, or a goat anti-human IL-1β, TNF-α, or PDGF polyclonal IgG at 20 μg (R&D Systems, Minneapolis, MN) per ml.

Techniques: Expressing

Journal: The Journal of investigative dermatology

Article Title: Cytokine expression is downregulated by collagen-polyvinylpyrrolidone in hypertrophic scars.

doi: 10.1046/j.1523-1747.1998.00329.x

Figure Lengend Snippet: Figure 11. Fibroblasts derived from HSc treated with collagen-PVP were assayed for collagen synthesis and cytokine expression. The percentage of relative collagen was determined by [14C]-Proline incorporation, values were compared between treated and untreated groups, but they were not significantly different. Results represent the mean 6 SD of triplicates of each culture (a). TGF-β1 and PDGF-AB levels were evaluated by enzyme- linked immunosorbent assay. Results represent the mean 6 SD of two different experiments performed by duplicate. Where p values for TGF-β1 are ø0.05 for normal skin or HSc versus HSc treated, and ø0.003 for PDGF-AB for normal skin or HSc treated versus HSc (b), normal skin, HSc, and collagen- PVP-treated HSc.

Article Snippet: Immunohistochemical procedures were assessed by blocking with 3% egg albumin (Sigma), except for the TGF-β1 assay in which the blockade was done with 3% bovine albumin (Sigma), and then the sections were incubated with a mouse anti-human E-selectin (ELAM-1) or vascular cell-adhesion molecule (VCAM-1) monoclonal IgG at 25 μg (Genzyme, Cambridge, MA) per ml, or a goat anti-human IL-1β, TNF-α, or PDGF polyclonal IgG at 20 μg (R&D Systems, Minneapolis, MN) per ml.

Techniques: Derivative Assay, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner

doi: 10.1038/s41419-020-03054-z

Figure Lengend Snippet: a A heat map generated using the significantly changed genes categorized in the “cytokine-cytokine receptor interaction pathway” is shown. b , c mRNA expression ( b ) was evaluated by qRT-PCR and protein concentration by ELISA ( c ) in CM of RASSF1A-overexpressing CNE-2 cells, RASSF1A-depleted CNE-1 cells and their corresponding control cells, The data are presented as the mean ± S.D. values, ** p < 0.01, Student’s t test. d – g PDGFB was transiently knocked down with a pool of siRNA or treated with a neutralizing antibody for PDGF-BB (10 µg/mL) in RASSF1A-depleted CNE-1 cells. PDGF-BB secretion in the CM was measured by ELISA ( d ), ** p < 0.01, Student’s t test. e Number of spheroids formed was determined via microscopy, and representative images ( e left panel) are shown. The formed spheroids were compared ( e right panel), the data are presented as the mean ± S.D. values, * p < 0.05, ** p < 0.01, Student’s t test; ns: non-sinificant. Scale bar: 200 µm. Representative images of the migration assay ( f ) and invasion assay ( g ) are shown, the data are presented as the mean ± S.D. values, ** p < 0.01, Student’s t test. Scale bar: 100 µm. h – j Recombinant PDGF-BB or IgG was added to RASSF1A-overexpressing CNE-2 cells. Representative images of sphere formation ( h ) (Scale bar: 200 µm.), migration ( i ) and invasion ( j ) assays (Scale bar: 100 µm) of RASSF1A-overexpressing CNE-2 cells treated with PDGF-BB (the culture medium was supplemented with 20 ng/ml or an equal volume of control IgG) are shown, The data are presented as the mean ± S.D. values, * p < 0.05, ** p < 0.01, Student’s t test.

Article Snippet: Cells were plated in 6-well plates (Corning, USA) and treated with humane recombinant PDGF-BB (220-BB-010, R&D Systems, USA) or Immunoglobulin G (IgG) control (AB-108-C, R&D Systems, USA) or neutralizing antibody against PDGF-BB (AB-220-NA, R&D Systems, USA) or latrunculin b (LTB, ab144291, Abcam, UK) 12 h after plating.

Techniques: Generated, Expressing, Quantitative RT-PCR, Protein Concentration, Enzyme-linked Immunosorbent Assay, Control, Microscopy, Migration, Invasion Assay, Recombinant

Journal: Cell Death & Disease

Article Title: RASSF1A inhibits PDGFB-driven malignant phenotypes of nasopharyngeal carcinoma cells in a YAP1-dependent manner

doi: 10.1038/s41419-020-03054-z

Figure Lengend Snippet: a – c YAP1 was transiently knocked down in RASSF1A-depleted CNE-1 cells. a In the indicated cells, YAP1 protein expression was assessed by using western blotting; b PDGFB, CYP61 and CTGF mRNA expression was assessed by qRT-PCR; c Concentration of PDGF-BB secreted in CM was measured by ELISA. The data are presented as the mean ± S.D. values, * p < 0.05, ** p < 0.01, Student’s t test. d – f Recombinant PDGF-BB or IgG was added to YAP1-silenced NPC cells. d The formed spheroids were counted via microscopy, and ( e ) representative images are shown. f The impact of PDGF-BB treatment on the migration and invasion of RASSF1A-depleted cells was determined by Transwell assays. The data are presented as the mean ± S.D. values, * p < 0.05, ** p < 0.01, Student’s t test. Scale bar: 200 µm.

Article Snippet: Cells were plated in 6-well plates (Corning, USA) and treated with humane recombinant PDGF-BB (220-BB-010, R&D Systems, USA) or Immunoglobulin G (IgG) control (AB-108-C, R&D Systems, USA) or neutralizing antibody against PDGF-BB (AB-220-NA, R&D Systems, USA) or latrunculin b (LTB, ab144291, Abcam, UK) 12 h after plating.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Microscopy, Migration

Journal: Circulation

Article Title: Platelet-derived growth factor-AB limits the extent of myocardial infarction in a rat model: feasibility of restoring impaired angiogenic capacity in the aging heart.

doi: 10.1161/hc0502.103672

Figure Lengend Snippet: Figure 3. A, Representative vWF (DAB) staining of vessels in 4- and 24-month-old rat hearts pretreated with vehicle or PDGF-AB 24 h before euthanization (magnification 200). B, Vascular density as determined by vWF staining for the sets of treated rats (number of vessels per high-power field [HPF; mag- nification 400]) (n3 for each set). *P0.05 vehicle vs control; vascular density differences between 4- and 24-month-old treat- ment sets are not statistically significant.

Article Snippet: In addition, at the time of cardiac or pulmonary allograft transplantation, sets of young adult mice also were treated with single subcutaneous pinnal injections of antibodies to neutralize PDGF-AB (10 g in 20 L PBS, AB-20-NA, R&D Systems, n 8 cardiac, 8 pulmonary allografts), PDGFR- , PDGFR- (10 g in 20 L PBS, AB-307-NA and AB385, R&D Systems, respectively; n 7 cardiac allografts), or nonimmune rabbit immunoglobulin G (10 g in 20 L PBS, AB-105-C, R&D Systems; n 8 cardiac, 8 pulmonary allografts).

Techniques: Staining, Control

Journal: Circulation

Article Title: Platelet-derived growth factor-AB limits the extent of myocardial infarction in a rat model: feasibility of restoring impaired angiogenic capacity in the aging heart.

doi: 10.1161/hc0502.103672

Figure Lengend Snippet: Figure 4. Representative Masson’s trichrome staining in 4-month-old rat hearts pretreated with PBS or PDGF-AB 24 h before LAD ligation. Graph shows myocardial infarct size scored 14 days after coronary artery ligation (control, n13; PDGF-AB, n12). *P0.02, PDGF vs control.

Article Snippet: In addition, at the time of cardiac or pulmonary allograft transplantation, sets of young adult mice also were treated with single subcutaneous pinnal injections of antibodies to neutralize PDGF-AB (10 g in 20 L PBS, AB-20-NA, R&D Systems, n 8 cardiac, 8 pulmonary allografts), PDGFR- , PDGFR- (10 g in 20 L PBS, AB-307-NA and AB385, R&D Systems, respectively; n 7 cardiac allografts), or nonimmune rabbit immunoglobulin G (10 g in 20 L PBS, AB-105-C, R&D Systems; n 8 cardiac, 8 pulmonary allografts).

Techniques: Staining, Ligation, Control

Journal: Circulation

Article Title: Platelet-derived growth factor-AB limits the extent of myocardial infarction in a rat model: feasibility of restoring impaired angiogenic capacity in the aging heart.

doi: 10.1161/hc0502.103672

Figure Lengend Snippet: Figure 5. Representative Masson’s trichrome staining in 24-month-old rat hearts pretreated with PBS or PDGF-AB 24 h before LAD ligation. Graph shows myocardial infarct size 14 days after coronary ligation (control, n5; PDGF-AB, n7). *P0.03, PDGF vs control.

Article Snippet: In addition, at the time of cardiac or pulmonary allograft transplantation, sets of young adult mice also were treated with single subcutaneous pinnal injections of antibodies to neutralize PDGF-AB (10 g in 20 L PBS, AB-20-NA, R&D Systems, n 8 cardiac, 8 pulmonary allografts), PDGFR- , PDGFR- (10 g in 20 L PBS, AB-307-NA and AB385, R&D Systems, respectively; n 7 cardiac allografts), or nonimmune rabbit immunoglobulin G (10 g in 20 L PBS, AB-105-C, R&D Systems; n 8 cardiac, 8 pulmonary allografts).

Techniques: Staining, Ligation, Control